tonebp (Santa Cruz Biotechnology)
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93
Structured Review
Santa Cruz Biotechnology
tonebp
Tonebp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nfat5/NFAT5+Antibody/pmc12933830-167-28-30
Average 93 stars, based on 98 article reviews
Tonebp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nfat5/NFAT5+Antibody/pmc12933830-167-28-30
Average 93 stars, based on 98 article reviews
tonebp - by Bioz Stars,
2026-10
93/100 stars
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Immunofluorescence:Article Title: Regulation of Microtubule Stability in Pulmonary Microvascular Endothelial Cells in Rats with Severe Acute Pancreatitis: Qingyi Decoction is a Potential CDK5 Inhibitor Article Snippet: .. Antibodies against immunofluorescence CDK5 (CAT# 14145) and Acetyl-alpha-Tubulin (CAT#35652) were purchased from CST (USA); Article Title: Regulation of Microtubule Stability in Pulmonary Microvascular Endothelial Cells in Rats with Severe Acute Pancreatitis: Qingyi Decoction is a Potential CDK5 Inhibitor Article Snippet: .. Antibodies against immunofluorescence CDK5 (CAT# 14145) and Acetyl-alpha-Tubulin (CAT#35652) were purchased from CST (USA); Incubation:Article Title: Heme Oxygenase-1 Suppresses Wnt Signaling Pathway in Nonalcoholic Steatohepatitis-Related Liver Fibrosis Article Snippet: 80 μ g/well proteins were loaded onto 10% SDS-PAGE for each sample, and proteins were transferred onto equilibrated polyvinylidene difluoride membranes (Millipore Corporation, Billerica, MA, USA) by electroblotting. .. Membranes were blocked with TBST containing 5% milk and incubated overnight at 4°C with primary antibodies against HO-1 (Bioworld Tech, LP, USA), Wnt1 (Abcam, Cambridge, MA, USA), Wnt5a (Novus Biologicals, Littleton, USA), β -catenin, GSK-3 β , phospho-GSK-3 β (Ser9) (ProteinTech Group, Chicago, USA), and Article Title: MicroRNA-20b carried by mesenchymal stem cell-derived extracellular vesicles protects alveolar epithelial type II cells from Mycobacterium tuberculosis infection in vitro. Article Snippet: Cells were lysed in mammalian protein extraction reagent (Thermo Fisher Scientific), and the lysates were loaded into micro-centrifuge tubes and centrifuged at 14,000 g for 10 min. Next, 30 μg protein extracts was boiled with 3-mercapto-1,2-propanediole (×4) (FUJIFILM Wako Pure Chemical) in sample buffer and boiled at 100 ◦C for 5 min. After that, the protein sample was run on 7% Mini-PROTEAN TGX to dissolve the gel (Bio-Rad Inc., Hercules, CA, USA) and transferred onto polyvinylidene fluoride membranes (Millipore Corp., Billerica, MA, USA). .. After being blocked in 5% non-fat milk for 1 h, the membranes were incubated with primary antibodies against GM130 (sc-55590, 1:2000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), TSG101 (ab83, 1:1000, Abcam Inc., Cambridge, MA, USA), CD81 (349502, 1:1500, BioLegend, San Diego, CA, USA), Article Title: NCOR1 maintains the homeostasis of vascular smooth muscle cells and protects against aortic aneurysm. Article Snippet: Phenotypic modulation of vascular smooth muscle cells (VSMCs) plays critical roles in the pathogenesis of aortic aneurysm (AA).. The function of nuclear receptor corepressor1 (NCOR1) in regulation of VSMC phenotype and AA is unclear.. Herein, using smooth muscle NCOR1 knockout mice, we demonstrated that smooth muscle NCOR1 deficiency decreased both mRNA and protein levels of contractile genes, impaired stress fibers formation and RhoA pathway activation, reduced synthesis of elastin and collagens, and induced the expression and activity of MMPs, manifesting a switch from contractile to degradative phenotype of VSMCs. Chromatin Immunoprecipitation:Article Title: The transcription factor NFAT5 limits infection-induced type I interferon responses Article Snippet: Samples were then incubated with 6 ng/μl RNase (Roche) for 30 min at 37°C, incubated for 1 h at 50°C after adding 350 μg/ml of Proteinase K (Roche), and DNA was purified by ethanol precipitation. .. Antibodies used for ChIP were the following: Control:Article Title: The transcription factor NFAT5 limits infection-induced type I interferon responses Article Snippet: Samples were then incubated with 6 ng/μl RNase (Roche) for 30 min at 37°C, incubated for 1 h at 50°C after adding 350 μg/ml of Proteinase K (Roche), and DNA was purified by ethanol precipitation. .. Antibodies used for ChIP were the following: Immunoprecipitation:Article Title: NCOR1 maintains the homeostasis of vascular smooth muscle cells and protects against aortic aneurysm. Article Snippet: Phenotypic modulation of vascular smooth muscle cells (VSMCs) plays critical roles in the pathogenesis of aortic aneurysm (AA).. The function of nuclear receptor corepressor1 (NCOR1) in regulation of VSMC phenotype and AA is unclear.. Herein, using smooth muscle NCOR1 knockout mice, we demonstrated that smooth muscle NCOR1 deficiency decreased both mRNA and protein levels of contractile genes, impaired stress fibers formation and RhoA pathway activation, reduced synthesis of elastin and collagens, and induced the expression and activity of MMPs, manifesting a switch from contractile to degradative phenotype of VSMCs. Magnetic Beads:Article Title: NCOR1 maintains the homeostasis of vascular smooth muscle cells and protects against aortic aneurysm. Article Snippet: Phenotypic modulation of vascular smooth muscle cells (VSMCs) plays critical roles in the pathogenesis of aortic aneurysm (AA).. The function of nuclear receptor corepressor1 (NCOR1) in regulation of VSMC phenotype and AA is unclear.. Herein, using smooth muscle NCOR1 knockout mice, we demonstrated that smooth muscle NCOR1 deficiency decreased both mRNA and protein levels of contractile genes, impaired stress fibers formation and RhoA pathway activation, reduced synthesis of elastin and collagens, and induced the expression and activity of MMPs, manifesting a switch from contractile to degradative phenotype of VSMCs. |
